human native ldl  (Lee Biosolutions)

Journal: medRxiv

Article Title: Gene-gene interactions protect against Familial Hypercholesterolemia: effect of lost- of-function PCSK9 variants

doi: 10.64898/2026.03.26.26348145

Figure Lengend Snippet: Pedigree of the family showing segregation of LDLR and PCSK9 variants. Squares represent males, circles are females and diamond symbols are used to represent family members without specifying their sex. Individuals fulfilling the clinical diagnosis criteria for familial hypercholesterolemia (FH) are indicated by half-filled symbols. The first row below each symbol correspond to the individual ID following by the genotype at LDLR, PCSK9 and APOB ..

Article Snippet: Human LDLR cDNA constructs were generated using a GFP-tagged plasmid vector (C-GFPSpark®, NM_000527; Sino Biological, Beijing, China).

Techniques: Biomarker Discovery

Journal: medRxiv

Article Title: Gene-gene interactions protect against Familial Hypercholesterolemia: effect of lost- of-function PCSK9 variants

doi: 10.64898/2026.03.26.26348145

Figure Lengend Snippet: Functional characterization of LDLR c.2479G>A ; (A) Flow cytometry histogram overlay showing DiI-LDL binding; (B) Quantification of LDL uptake relative to WT; (C) LDLR expression. The upper line represents the benignity cutoff (90%) established by the ACMG/ClinGen guidelines for functional studies. * <0.05; **** <0.0001.

Article Snippet: Human LDLR cDNA constructs were generated using a GFP-tagged plasmid vector (C-GFPSpark®, NM_000527; Sino Biological, Beijing, China).

Techniques: Functional Assay, Flow Cytometry, Binding Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Twenty-Four-Month rhGH Intervention: Insights into Redox Regulation, Vascular Biomarkers, and Body Composition in Adult GHD Patients

doi: 10.3390/ijms27031451

Figure Lengend Snippet: Longitudinal changes in ox-LDL, Trx, E-selectin, P-selectin, ICAM-1, and VCAM-1 during 24-month rhGH therapy at baseline, 12 months, and 24 months. Statistical significance is indicated by asterisks: * p < 0.05 vs. baseline; *** p < 0.001 vs. baseline; **** p < 0.00001 vs. baseline.

Article Snippet: OS and endothelial markers were quantified using commercially available enzyme-linked immunosorbent assay (ELISA) kits according to the manufacturers’ instructions: ox-LDL (Novus Biologicals, Bio-Techne, NBP3-18794; Minneapolis, MN, USA), Trx (XpressBio, XPEH0291; Frederick, MD, USA), OGG1 (Cloud-Clone Corp., SEC704Hu; Wuhan, China), VCAM-1 (Cloud-Clone Corp., SEA547Hu; Wuhan, China), ICAM-1 (Cloud-Clone Corp., SEA548Ca; Wuhan, China), and E-selectin (Cloud-Clone Corp., SEA029Hu; Wuhan, China).

Techniques:

Journal: bioRxiv

Article Title: Mast cell-driven adipocyte lipolysis promotes post-expansive VAT atrophy and ectopic hepatic steatosis

doi: 10.64898/2026.01.07.698076

Figure Lengend Snippet: ( a ) Heatmap of MC mediator-related gene expression in EAT in WT mice at week 0 and at week 10 of HFD feeding. n=3/ea. ( b ) Real-time PCR analysis of MC mediator-related genes Mcpt4 (encodes mouse mast cell protease 4 (mMCP-4), Cma1 (encodes mMCP-5), Hdc (encodes histidine decarboxylase), Ext1 (encodes exostosin-1), Ext2 , Ndst1 (encodes N-deacetylase/N-sulphotransferase 1), Ndst2 , Tph1 (encodes tryptophan hydroxylase 1), and Hpgds (encodes hematopoietic prostaglandin D synthase) in EAT in WT mice at week 0, 10, 20 of HFD feeding. n=6-8/ea. ( c/d ) Serum ( c ) and EAT ( d ) 5-HT levels in HFD-fed WT mice. n=6-8/ea. ( e ) EAT 5-HT levels in WT mice received i.p. injections of saline or DSCG from week 0 to 20 of HFD feeding. n=6-8/ea. ( f ) Real-time PCR analysis of Tph1 mRNA levels in adipocytes, SVFs, MC-depleted SVFs, and MCs from WT EAT in HFD-fed mice for 16 weeks. n=5-8/ea. ( g ) 5-HT + (red) and CD117 + (green), 5-HT + (red) and FceRI + (green) immunofluorescent double staining in WT EAT in HFD-fed mice for 16 weeks. ( h ) Correlations between serum LDL and 5-HT levels, IgE and 5-HT levels, LDL and NEFA levels, IgE and NEFA levels, and 5-HT and NEFA levels during EAT atrophy in HFD-fed mice. n=27. ( i ) Correlations between serum LDL and 5-HT levels, IgE and 5-HT levels, LDL and NEFA levels, IgE and NEFA levels, and 5-HT and NEFA levels in MASLD patients. n=50. ( j - l ) Differentiated 3T3-L1 adipocytes were treated with or without live BMMCs, BMMC lysates, LDL, LDL-activated BMMCs, DSCG- and LDL-treated BMMCs, or 5-HT. n=3/ea. ( j ) Representative images of oil-red O staining, media glycerol and NEFA levels in 3T3-L1 adipocytes. Scale bars: 50 µm. ( k/l ) Immunoblot analysis ( k ) and quantification of ( l ) ATGL, HSL and SIRT1 relative to β-actin, p-HSL(565) and p-HSL(563) relative to HSL, and p-AMPKα relative to AMPKα in 3T3-L1 adipocytes. Data are mean ± SEM. Pearson’s correlation test for h and i . P < 0.05 was considered significant correlation. Mann-Whitney U test for e . Kruskal-Wallis H test with Dunn’s post hoc adjustment for b-d , and f . Welch’s t-test for j and l . * p < 0.05, ** p < 0.01, *** p < 0.001. Different letters indicate statistically significant difference.

Article Snippet: To activate BMMCs, the cells were treated with 1 mg/mL LDL (Lee Biosolutions, Inc, Maryland Heights, MO Cat# 360-10).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Histone Deacetylase Assay, Saline, Double Staining, Staining, Western Blot, MANN-WHITNEY